Journal: Carcinogenesis

Article Title: Suppressive effects of nobiletin on hyperleptinemia and colitis-related colon carcinogenesis in male ICR mice.

doi: 10.1093/carcin/bgn080

Figure Lengend Snippet: Fig. 7. Proposed schema of molecular mechanisms by which NOB suppresses leptin secretion from 3T3-L1 cells. Differentiation and protein translation are regulated by both Akt/mTOR and MEK/ERK-signaling pathway. mTOR and the downstream factors, such as S6 and eIF4B, are phosphorylated in a constitutive manner. Rapamycin inhibits mTOR and thereby suppressing the activity of downstream molecules for blocking leptin secretion. Meanwhile, NOB induces the dephosphorylation of MEK1/2 without affecting mTOR and Raf and reduces phosphorylation of eIF4B.

Article Snippet: Antibodies directed against Pi-mitogen-activated protein kinase/extracellular signaling-regulated protein kinase kinase (MEK)1/2 (Ser217/221, #9121), Pi-extracellular signaling-regulated protein kinase (ERK)1/2 (Thr202/Tyr204), Pi-mammalian target of rapamycin (mTOR) (Ser2448, #2971), Pi-S6 (Ser240/244, #2215), Pi-eukaryotic initiation factor 4B (eIF4B) (Ser422, #3591), as well as horseradish peroxidase-conjugated anti-rabbit antibody (#7074), were obtained from Cell Signaling Technology (Beverly, MA).

Techniques: Activity Assay, Blocking Assay, De-Phosphorylation Assay, Phospho-proteomics

Journal: Biomedicines

Article Title: A Touchscreen Device for Behavioral Testing in Pigs

doi: 10.3390/biomedicines10102612

Figure Lengend Snippet: List of physical components and pricing as of purchase. Sums in each category are given in bold. * The board and components were purchased as a set of 5 to meet minimum order thresholds; price given is per board. ** A smaller rack would have been suitable even for the large Yorkshire pigs. Future builds will use the SRK-12 (12U height). *** The rack shelf was not explicitly necessary, but gave easy access to the Raspberry Pi.

Article Snippet: , Raspberry Pi 4B (2×) , $110 , SparkFun.

Techniques: